Restoration, maintaining and improvement of skills and physiological and psychological capacities is depending on regulative function of the autonomic nervous system (ANS) with its parasympathetic (inhibitory) and sympathetic (excitatory) subdivisions. To prevent, diagnose and treat autonomic imbalances like parasympathetic or sympathetic overtraining, parameters of ANS are of extensive interest to assess changes or dysfunction in relation to external load. Some parameters of heart rate variability (HRV) were sensitive to parasympathetic activity. Electrodermal activity (EDA) is solely sympathetically controlled and may supplement HRV. Actually, a combined consideration is missing in research on regeneration in sports and exercise. An explorative multidimensional investigation using a wireless multisensor combining both excitatory and inhibitory measures was applied in four subprojects in (elite) sports to assess individual internal load by parameters of ANS. External load was subsequently associated with reductions in parasympathetic activity (HRV) and increased sympathetic activity (EDA). Sympatho-vagal ratio was affected by external load independently of intensity. Moreover, nocturnal parasympathetic and sympathetic activity was individually affected by physiological and psychological stressors. No general reciprocal interaction between sympathetic and parasympathetic activity was found.A multidimensional analysis of ANS using parameters of HRV and EDA could be helpful in the future to elucidate physiologic coping strategies, regeneration and training control in relation to health and performance in sports and exercise. Because of high intra- and interindividual variability individual standardization and long-term monitoring seems to be essential.