In order to get knowledge about the contamination of vegetables by antibiotics and resistant bacteria, cabbage and leek were cultivated in two cultivation periods under conventional agricultural methods on experimental plots (South Westphalia University of Applied Sciences, Soest).The excrements for fertilization were spiked separately with enrofloxacin (ENR), tetracycline (TC), amoxicillin (AMO) or ceftiofur (CEF), each 75 mg/kg. Furthermore, extended-spectrum--lactamases (ESBL) producing and fluorchinolonresistant (PMQR) Escherichia coli (10 cfu/ml) were added to the manure. Analytical methods (LC/MS) were developed to determine extractable amounts of antibiotics (active compounds and metabolites) in various organs of harvested plants, soil and excreta. In addition, microbiological investigations were carried out (Freie Universität Berlin).The content of antibiotic residues in the vegetables was generally low. In leek and cabbage ENR- concentrations up to 0.3 g/kg fresh weight (fw) could be measured. The fertilization with tetracyclin-spiked animal excrements resulted in 5 g/kg fw in young leaves of leek. Vegetable plants of both cultivation periods contained no detectable -lactam-residues. Nevertheless, specially tailored evaluations of full-MS-data indicate products of degradation and conversion of AMO and CEF in plants, soil and manure. The microbiological studies lead to several positive findings of ESBL/PMQR-Enterobacteriaceae in plant and soil. As a consequence of the results of this study, the distribution of both antibiotic active compounds and multi-resistant populations from animal husbandry via nutrition plants into the food chain might be possible.