TY  - JOUR
AB  - The specific binding of DNA origami nanostructures (DONs) to bacteria is an important prerequisite for their application in pathogen targeting and antimicrobial drug delivery. So far, targeting bacteria with DONs has been achieved exclusively via aptamers, which suffer from drawbacks such as sensitivity toward environmental conditions and reduced binding after immobilization or conjugation. Here, an alternative approach is presented based on the modification of DONs with the cell wall-binding glycopeptide antibiotic vancomycin. Using strain-promoted azide-alkyne cyclo addition, azide-modified vancomycin is conjugated to selected staple strands and subsequently incorporated into 2D DON triangles. The resulting constructs show specific binding to the Gram-positive species Bacillus subtilis (B. subtilis) and Staphylococcus capitis (S. capitis), and remarkably, to Gram-negative Escherichia coli (E. coli), but no antimicrobial activity at vancomycin concentrations up to at least 2.91 μM. For B. subtilis and E. coli, DONs with vancomycin modifications on both sides exhibit better binding than DONs modified on only one side. However, both variants bind equally well to S. capitis. These results demonstrate the great potential of small molecule drug compounds for the robust, broad-spectrum targeting of bacteria with DONs.Targeting a ubiquitous cell wall component of most pathogenic bacteria, vancomycin-modified DONs have many potential applications in the prevention and treatment of nosocomial infections.
AU  - Leineweber, Özge Coşkuner
AU  - Pothineni, Bhanu K.
AU  - Schumann, Nils
AU  - Hofmann, Ulrike
AU  - Möser, Christin
AU  - Smith, David M.
AU  - Grundmeier, Guido
AU  - Zhang, Yixin
AU  - Keller, Adrian
DO  - 10.17619/UNIPB/1-2472
PB  - Universitätsbibliothek
DP  - Universität Paderborn
LA  - eng
PY  - 2025
SN  - 2688-4062
SP  - 1 Online-Ressource (Seite 1-10) : Illustrationen, Diagramme
T2  - Small Structures
TI  - Vancomycin-Modified DNA Origami Nanostructures for Targeting Bacterial Pathogens
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:466:2-56945
Y2  - 2026-01-12T02:13:09
ER  -